Applications Notes and Pulse Sequences for Discovery and Apollo consoles
Notes...
We welcome submissions from users.
The ZIP archive contains the application note in Adobe pdf file format and the NTNMR data file/pulse sequence.
For single resonance systems, e.g. (Apollo/Discovery HF1/LF1) download the single resonance ZIP archive where specified.
Tecmag users with multiple resonance systems, e.g. (Apollo/Discovery HF2/HF3/HF4) must download the ZIP archive for multiple resonance spectrometers.
Warning!!! DO NOT DOWNLOAD THESE SEQUENCES UNLESS YOU HAVE AT LEAST VERSION 2.0 OF NTNMR. Check the version of NTNMR by going to the Help|About menu. To update an older version of NTNMR, please contact Tecmag's support group at
COSY (Magnitude) The COSY pulse sequence generates a 2D NMR spectrum that correlates homonuclear-coupled nuclei. The basic COSY technique with phase cycling for magnitude processing, 2D NMR data acquisition setup and processing on Tecmag spectrometers is described. An NMRscript is provided for 2D magnitude data processing.
COSY (Phase Sensitive - States) The basic procedure of 2D States (also called States-Haberkorn-Ruben, RuSH, or Hypercomplex) phase sensitive NMR data acquisition and processing on Tecmag spectrometers is described. An NMRscript is included for 2D States data processing.
COSY (Phase Sensitive - TPPI) This experiment incorporates the Time-Proportional receiver Phase Incrementation (TPPI) technique in the COSY pulse sequence to yield a pure absorption phase 2D spectrum. The basic procedure of 2D TPPI phase sensitive NMR data acquisition and processing on Tecmag spectrometers is described. An NMRscript for the TPPI 2D data processing is provided.
CPMAS with TPPM Decoupling A Two Pulse Phase Modulation (TPPM) proton decoupling scheme is applied after cross-polarization from protons to a rare spin.
DEPT-135
The Distortionless Enhancement by Polarization Transfer (DEPT) experiment uses polarization transfer from protons to a X-nucleus to increase signal strength. The DEPT-135 sequence produces 13C-decoupled spectra with the methine (CH) and methyl (CH3) carbons phased upward while the methylene (CH2) carbons are downward.
DEPT-Spectral Editing
The spectral editing DEPT experiment acquires three raw DEPT spectra. While the CH spectrum can be directly obtained as one of the raw spectra, an NMRscript generates the CH2 and CH3 spectra using the three raw spectra.
HETCOR
The 2D C, H correlation experiment shows cross signals for all directly bonded 1H to 13C nuclei. The basic heteronuclear chemical shift correlation (HETCOR) experiment with phase cycling for magnitude processing, the procedure of 2D NMR data acquisition and processing is described. An NMRscript is provided for the 2D magnitude data processing.
INEPT
The Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) experiment increases the signal strength for nuclei with a low gyromagnetic ratio by transferring polarization from protons via the H,X spin coupling.
One Pulse NMR with WALTZ-16 Decoupling
The WALTZ-16 is introduced to demonstrate the implementation of a composite pulse decoupling sequence. The WALTZ-16 sequence is applied as a subsequence. In NTNMR the Asynchronous function conveniently merges a subsequence into the main sequence.
REDOR
Rotational-Echo Double-Resonance nuclear magnetic resonance (REDOR) is a magic-angle spinning experiment for measuring internuclear distances between heteronuclear spin pairs through the dipolar interaction.
SELTICS
The SELTICS (Sideband Elimination by Temporary Interruption of Chemical Shift) sequence suppresses spinning sidebands through multipulse narrowing of the chemical shift scale.
T1rho Measurements
The T1rho experiment measures the relaxation time in the rotating frame. Two experiments are included: a single-resonance version where nuclei are excited by a 90-degree pulse and then spin-locked and a double-resonance where the rare spin is excited by cross-polarization from protons and then spin-locked
T2 Measurements (I)
The Carr-Purcell-Meiboom-Gill (CPMG) method is applied to measure the transverse or spin-spin relaxation time T2. With this sequence, the FID's are directly used to calculate T2.
T2 Measurements (II)
The Carr-Purcell-Meiboom-Gill (CPMG) method is applied to measure the transverse or spin-spin relaxation time T2. This sequence has more general applications compared to the one in T2 Measurements (I).
TOSS
Total Suppression of Spinning Sidebands (TOSS) is achieved by using a series of 180-degree pulses at carefully determined points in a rotor cycle after excitation (initial 90 degree pulse or cross polarization).
Notes...
